Laboratory Practice - Paracetamol in Drugs

These practical kits allow students with or without electrochemical experience to complement their academic
programs by an inquiry-based learning on research experiences. They are also useful for those researchers that are starting in the biosensing field.

The determination method is based in the electrochemical oxidation of acetaminophen to N-acetyl-p-benzoquinone imine. The identification of the reduction-oxidation process is done by CV that also allows to define if the process is controlled by adsorption or by diffusion. After process characterization, Cyclic Voltammetry and Square Wave Voltammetry parameters and analytical characteristics of both methods are studied and defined. Comparison of the results obtained with both electrochemical methods can be done by determination of paracetamol in pharmaceutical preparations by direct quantification or standard aditions. Also, Amperometric Detection (AD) is optimized in a flow-injection analysis system, defining the hydrodynamic curve and allowing the measurement of different samples under flow conditions.

The kit contains:

• The professor outline
• The student outline

• A box of suitable screen-printed electrodes (50 units)

• The needed analyte

These practical kits allow students with or without electrochemical experience to complement their academic
programs by an inquiry-based learning on research experiences. They are also useful for those researchers that are starting in the biosensing field.

The determination method is based in the electrochemical oxidation of acetaminophen to N-acetyl-p-benzoquinone imine. The identification of the reduction-oxidation process is done by CV that also allows to define if the process is controlled by adsorption or by diffusion. After process characterization, Cyclic Voltammetry and Square Wave Voltammetry parameters and analytical characteristics of both methods are studied and defined. Comparison of the results obtained with both electrochemical methods can be done by determination of paracetamol in pharmaceutical preparations by direct quantification or standard aditions. Also, Amperometric Detection (AD) is optimized in a flow-injection analysis system, defining the hydrodynamic curve and allowing the measurement of different samples under flow conditions.

The kit contains:

• The professor outline
• The student outline

• A box of suitable screen-printed electrodes (50 units)

• The needed analyte