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AN-P-064

Separation of sugars and sugar acids using low-pressure gradient


Summary

A low-pressure gradient enables the separation of sugar acids strongly retained on the column and sugars within an appropriate analysis time. The saccharides are separated on a column of the Metrosep Carb 2 - 250/4.0 type with subsequent pulsed amperometric detection (PAD). Galactose and arabinose are not completely separated under the selected conditions.

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Metrohm Nordic AS

Sandviksveien 26
1363 Høvik

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