AN-U-055
Amino acids in cell culture growth medium applying UV/VIS detection after post-column reaction with ninhydrin at 120 °C
Summary
Cell culture growth media contain all required components to keep cells alive. Here the amino acid composition is analyzed. A binary gradient separates in this example amino acids. The post-column reaction with ninhydrin requires a temperature of 120 °C, while the samples need to be cooled for stability.