Laboratory Practice - Ascorbic Acid in Juice

These practical kits allow students with or without electrochemical experience to complement their academic
programs by an inquiry-based learning on research experiences. They are also useful for those researchers that are starting in the biosensing field.

Most of the methods described for ascorbic acid determination are based on the ascorbic acid oxidation, therefore Cyclic
Voltammetry (CV) is used to identify the oxidation process over screen-printed electrodes. CV is a powerful technique also to determine if the process is controlled by adsorption or diffusion and study the effect of pH variation on the ascorbic acid electrochemical behaviour.
On a second step, Differential Pulse Voltammetry (DPV) is used for developing an electrochemical method for ascorbic acid detection. Its analytical characteristics are determined and measurement of the analyte in a real sample is carried out in batch and in flow-injection analysis.

The kit contains:

• The professor outline
• The student outline

• A box of suitable screen-printed electrodes (50 units)

• The needed analyte

These practical kits allow students with or without electrochemical experience to complement their academic
programs by an inquiry-based learning on research experiences. They are also useful for those researchers that are starting in the biosensing field.

Most of the methods described for ascorbic acid determination are based on the ascorbic acid oxidation, therefore Cyclic
Voltammetry (CV) is used to identify the oxidation process over screen-printed electrodes. CV is a powerful technique also to determine if the process is controlled by adsorption or diffusion and study the effect of pH variation on the ascorbic acid electrochemical behaviour.
On a second step, Differential Pulse Voltammetry (DPV) is used for developing an electrochemical method for ascorbic acid detection. Its analytical characteristics are determined and measurement of the analyte in a real sample is carried out in batch and in flow-injection analysis.

The kit contains:

• The professor outline
• The student outline

• A box of suitable screen-printed electrodes (50 units)

• The needed analyte