AN-U-054
Amino acids applying UV/VIS detection after post-column reaction with ninhydrin at 120 °C
Resumo
The determination of amino acid is an important task in pharmaceutical and biochemical applications. A binary gradient separates in this example 17 amino acids of a commercially available standard solution. The post-column reaction with ninhydrin requires a temperature of 120 °C, while the samples need to be cooled for stability.